22 апреля 2025
A New Method for Accelerated Aging of Nanoparticles to Assess the Colloidal Stability of Albumin-Coated Magnetic Nanoparticles
Nikolaev, B.; Yakovleva, L.; Fedorov, V.; Yudintceva, N.; Tarasova, D.; Perepelitsa, E.; Dmitrieva, A.; Sulatsky, M.; Srinivasan, S.; Sonawane, S.H.; et al.
Nanomaterials 2025, 15, 475. https://doi.org/10.3390/ nano15070475
The colloidal long-storage stability of nanosized drugs is a crucial factor for
pharmacology, as they require much time for robust estimation. The application of bioavailable magnetic nanosuspensions in theranostics is limited by incomplete information about
their colloidal stability in the internal media of human organisms. A method for the
accelerated temperature stress “aging” of magnetic nanosized suspensions is proposed
for the rapid assessment and prediction of the colloidal stability over time of nanosized
iron oxide suspensions stabilized by albumin HSA. Colloidal stability is assessed using
dynamic light scattering (DLS), fluorescence spectroscopy, electrophoresis, and ion monitoring methods during short- and long-term storage. Rapid assessment is achieved by
short high-temperature (70 ◦C) processing of carboxymethyl-dextran-coated nanosol in
the presence of albumin. The role of albumin in the sustained stability of superparamagnetic iron oxide particles (SPIONs) was studied under conditions mimicking blood plasma
(pH = 7.4) and endolysosomal cell compartments (pH = 5.5). According to the fluorescence
quenching and DLS data, colloidal stability is ensured by the formation of an HSA corona
on carboxymethyl-dextran-coated SPIONs and their process of clustering. In the presence
of albumin, the colloidal stability of nanoparticles is shown to increase from 80 to 121 days
at a storage temperature of 8 ◦C The prognostic shelf life of magnetic nanosol is estimated
by calculating the Van’t Hoff’s relation for the rate of chemical reactions. The validity
of using the Van’t Hoff’s rule is confirmed by the agreement of the calculated activation
energy at 8 ◦C and 70 ◦C. The developed method of the accelerated aging of nanoparticles
can not only be employed for the estimation of the shelf life of magnetic nanoparticles
coated with HSA in vitro but also for assessing the stability of SPIONs applied in vivo.